Proceedings of fluorescence detection IV 1-2 February 1996, San Jose, California

Cover of: Proceedings of fluorescence detection IV |

Published by SPIE in Bellingham, Wash .

Written in English

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Subjects:

  • Fluorescence -- Measurement -- Congresses.

Edition Notes

Book details

Other titlesFluorescence detection IV, Proceedings of fluorescence detection 4, Proceedings of fluorescence detection four
StatementE. Roland Menzel, chair/editor ; sponsored and published by SPIE--the International Society for Optical Engineering.
SeriesProgress in biomedical optics, SPIE proceedings series ;, v. 2705, Proceedings of SPIE--the International Society for Optical Engineering ;, v. 2705.
ContributionsMenzel, E. Roland, 1943-, Society of Photo-optical Instrumentation Engineers.
Classifications
LC ClassificationsQC477 .P86 1996
The Physical Object
Paginationvii, 200 p. :
Number of Pages200
ID Numbers
Open LibraryOL820462M
ISBN 100819420794
LC Control Number95072300

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PROCEEDINGS VOLUME Fluorescence Detection IV. Editor(s): E. Roland Menzel; Abraham Katzir Fluorescence detection and characterization of packing defects in lyotropic liquid crystals Author(s): Sun-Yung Chen; Kwan Hon Cheng Show Abstract.

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Fluorescence detection IV Proceedings of fluorescence detection 4 Proceedings of fluorescence detection four: Responsibility: E. Roland Menzel, chair/editor ; sponsored and published by SPIE--the International Society for Optical Engineering.

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We present recent progress in the field of sensing fluorescently labeled biomolecules, as separated by micro-chip capillary electrophoresis, being a method to improve the detection limit of the fluorescence signal by numerical post-processing and a way for parallel detection of differently labeled sets of molecules using en-coded excitation.

Immunoassays are, at present, an important tool for diagnostics, drug development, and environmental monitoring. However, most immunoassays involve procedures that require many elements for their development.

We introduce a novel biosensing platform based on fluorescence quenching caused by graphene oxide (GO) for the detection of Human-IgG and Prostate-Specific Antigen (PSA). Wavelength-selective monitoring of the on-chip separation of fluorescent dyes is implemented as a proof-of-principle.

We envision well-controlled microfluidic plug formation, waveguide excitation, and a low limit of detection to enable monitoring of extremely small quantities with high spatial resolution. SERRS is an extremely sensitive spectroscopic technique that offers several advantages over conventional fluorescence detection.

For example, the high sensitivity of the method allows detection of DNA capture from single plasmonic array "pixels" ∼1 μm 2 in area. Additionally, the information rich nature of the SERRS spectrum allows multiple. The detection of the fluorescence intensity takes place in a flow cytometer [19,20].

A sandwich procedure is also used in this assay. A sandwich procedure is also used in this assay. The difference to the sandwich ELISA is the use of a fluorescent dye instead of. While there was a substantial difference in the fluorescence penetration, the maximum fluorescence intensity in the brightest portions of the gel is similar in both the 10 min and 4 h time points at ± and ± × 10 4 arbitrary fluorescence units (AFUs), respectively (n = Proceedings of fluorescence detection IV book.

This result is consistent with transport. Published in SPIE Proceedings Vol. Fluorescence Detection IV E. Roland Menzel; Abraham Katzir, Editor(s). optical biopsy iv proceedings of spie Posted By Kyotaro Nishimura Media Publishing TEXT ID Online PDF Ebook Epub Library point measurement that is we propose a method for computer assisted optical biopsy for colorectal polyps with the.

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Laser-induced fluorescence (LF) has been developed as a diagnostic tool for the detection of atherosclerosis. We have examined the use of LF for the identification of accelerated atherosclerotic plaque growth induced by Marek's Disease Virus (MDV) infection in White Leghorn rooster chicks (R) as well as plaque regression after treatment.

Time-Resolved Laser Spectroscopy in Biochemistry IV (Proceedings of SPIE, Vol. By JR Lakowicz (Editors). By JR Lakowicz (Editors). Abstract, Publisher. fluorescence detection ii proceedings of spie Posted By David Baldacci Media Publishing TEXT ID c45de Online PDF Ebook Epub Library spectral region the porphyrin producing bacterium propionibacterium acnes which is involved in the pathogenesis of acne vulgaris was localized in human skin spectrally.

CONFERENCE PROCEEDINGS Papers Presentations Journals. Advanced Photonics Journal of Applied Remote Sensing Journal of Astronomical Telescopes, Instruments, and Systems Journal of Biomedical Optics Journal of Electronic Imaging Journal of Medical Imaging Journal of Micro/Nanolithography, MEMS, and MOEMS.

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Fluorescence is the phenomenon of the emission of a light quanta by a molecule or material (fluorophore) after initial electronic excitation in a light-absorption process. After excitation, a molecule resides for some time in the so-called excited state and its fluorescence emission can be observed usually with a lower energy (longer wavelength) than the excitation.

advanced biomedical and clinical diagnostic systems iv proceedings of spie Posted By Enid BlytonMedia Publishing TEXT ID d8 Online PDF Ebook Epub Library ADVANCED BIOMEDICAL AND CLINICAL DIAGNOSTIC SYSTEMS IV PROCEEDINGS.

The specific hypothesis is that fluorescence lifetime can be employed as reliable contrast parameter for providing higher detection sensitivity as compared with conventional intensity-based tumor imaging approaches and therefore it is possible to detect smaller tumor volumes (early detection) than those achieved by other prevailing methods.

Other chapters deal with fluorescence-related topics, such as the presentation in Chap. 13 (by Chris Zu and Warren Zipfel) of multiphoton excitation of fluorescence probes and practical techniques for estimating the excitation cross section of dyes; Chap.

19 (by Sebastian Wachsmann-Hogiu and Daniel Farkas) of multispectral and fluorescence. The technological advancements made in optics and semiconductors (e.g., cameras and laser diodes) working with infrared have brought interest in optical bioimaging back to the forefront of research investigating in vivo medical imaging techniques.

The definition of the near-infrared transparency windows has turned optical imaging into more than just a method for topical imaging. Principles of Fluorescence Detection.

Albani, J.R., Absorption et Fluorescence: Principes et Applications, Lavoisier (). This book is the first on absorption and fluorescnece to be published in the French language.

Albani, J.R., Principles and Applications of Fluorescence Spectroscopy, Wiley-Blackwell (). Get this from a library. Proceedings of advances in fluorescence sensing technology IV: JanuarySan Jose, California.

[Joseph R Lakowicz; Steven A Soper; Richard B Thompson, (Associate professor of biochemistry); Society of Photo-optical Instrumentation Engineers.; International Biomedical Optics Society.; Coherent Laser Group.;].

Optical detection has also been used in microfluidic immunoassays, such as fluorescence detection 7, 15, 20, 21, 33, 34 using diode lasers coupled with PMT and appropriate wavelength filters.

Examples of chemiluminescence detection by the use of charged coupled device (CCD) cam 25, 35 and PMT 36 can also be found in the literature.

Dipeptidyl peptidase-IV (DPP-IV), an important prolyl-specific peptidase in the serine hydrolase family, hydrolyzes a large number of endogenous oligopeptides.

In clinical studies, the detection of DPP-IV, especially the detection of its activity, is particularly important. We previously reported a specific fluorescent two-photon probe for DPP-IV. Introduction. Fluorescence technology, since its introduction to biochemistry in the s, has offered an important analytical tool for both biological research and clinical applications in biological molecules [], DNA microarray technology [], cell imaging [], cancer diagnosis [] and so on [].Due to its high sensitivity and selectivity, fluorescence detection has undergone enormous.

“Two-photon fluorescence biosensing with conventional and photonic crystal fibers”, Optical fibers and sensors for medical applications IV, SPIE, vol.().

Zhody MJ, Tse C, Ye JY, and O’Donnell M, “An ultrasonic method to measure effective temperature in the vicinity of laser-induce optical breakdown”, Proceedings of.

It summarizes the results of the 5th Fluorescence Workshop, which was held in El Escorial, Spain from September 16th to 20th, After a short overview on the principle of the detection of air showers using fluorescence light (Section ), experiments applying this technique to register cosmic-ray induced air showers are described (Section.

Radio detection promises measurements with high duty-cycle, allows a direction reconstruction with very good angular resolution, and provides complementary information on energy and nature of the cosmic ray primaries with respect to particle detectors at ground and fluorescence .

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